Trans-Aconitate Utilization by Sheep

Inhibition of aconitase by trans-aconitate.

Bernheim (2), studying the properties of the “citric dehydrogenase” system, noted that aconitic acid (presumably the trans isomer) inhibited the reduction of methylene blue by citric acid. More recently, Morrison and Still (3), in a short report, claimed that truns-aconitic acid inhibited competitively the reaction cis-aconitate c citrate, catalyzed by the aconitase of rhubarb leaf. The present...

Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferases.

The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further demonstrate that the product is the 6-methyl ester of trans-aconitate (E-3-car...

Microbiological Dissimilation of Tricarballylate and Trans-aconitate.

Altekar, W. W. (National Chemical Laboratory, Poona, India) and M. R. Raghavendra Rao. Microbiological dissimilation of tricarballylate and trans-aconitate. J. Bacteriol. 85:604-613. 1963.-Two fluorescent pseudomonads capable of metabolizing tricarballylate and trans-aconitate were isolated by the soil-enrichment culture technique. These and some other species of bacteria were tested for their ...

trans-aconitate metabolism in a riboflavin producing

Acetate utilization in sheep.

Acetate, an important metabolite in most species, has a unique place in ruminant metabolism. As the major product of ruminal and caecal fermentation, acetate provides much of the energy requirements of ruminant tissues. Little information is available, however, on the overall utilization of acetate in sheep. Intravenous-acetate tolerance tests in sheep (Jarrett, Potter & Filsell, 1952; Pugh & S...